InstiGRO™: INCREASED CLONING EFFICIENCY & EFFICACY

InstiGRO™-CHO and -HEK supplements dramatically improve cell growth and colony formation in limiting dilution and semi solid media cloning.

Features:

  • Supplied as an easy to use 20X stock solution.
  • Supplied in vials of 5ml as standard.
  • Animal derived component free.

Benefits:

  • Dramatically enhanced cloning efficiency.
  • Accelerated post-seeding growth kinetics.
  • Reduced workload.
  • Greater cell survival increases probability of isolating high producers.

Cell Growth Under Limiting Dilution

InstiGRO verus media

CHO DHFR-ve cells cultured in defined media (HyClone™ CDM4CHO™) were plated at 5 cells per well in 96 well plates and monitored for 15 days.

Cell growth to at least 10% confluence was observed in 96% of wells in the presence of InstiGRO, compared to 9% of wells in the presence of recombinant albumin and 1% with no supplement.

Cells reached 80% confluence in 88% of wells in the presence of InstiGRO™, 2% of wells in the presence of recombinant albumin and no wells in media alone.



Colony Formation in Semi Solid Media

InstiGRO verus media

HEK cells cultured in defined media were plated in HEK-semi solid media with and without InstiGRO™ HEK at 1500 cells/ml and cultured for 12 days.Colonies formed with greater efficiency in the presence of InstiGRO, reducing the optimal seeding density by 66%.

About

FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.

Product Codes

InstiGRO™ CHO IGR1101
InstiGRO™ HEK IGR1501

Please contact us to request a sample, enquire about pricing, or to place an order.