Monoclonal Antibody Production and Hybridoma Services

Building on many years experience culturing hybridoma cells, we offer our customers a comprehensive range of services designed to maximise the efficiency of their antibody production workflows; encompassing both hybridoma optimisation and antibody production.

Cell Line Characterisation

  • Analysis of clone stability and homogeneity.
  • Analysis of cell line productivity by ELISA (data normalised to cell number).
  • Analysis of cell line growth kinetics.

Cell Line Optimisation

  • Re-cloning of heterogeneous cell lines - using clone productivity as the primary selection criteria.
  • Adaption to serum-free cell culture.
  • Media optimisation.

Cell Line Expansion and Antibody Production

  • Cell expansion.
  • Cell banking and controlled-rate cryopreservation.
  • Supernatant harvest.
  • Antibody purification.

Antibody Characterisation

  • Isotyping.
  • Sample analysis on SDS PAGE.
  • Real-time and accelerated stability testing.
  • Testing in end-point assays (Western, ELISA, Immunofluorescence, Plate Reader assays, etc).

Please contact us for pricing and to discuss your specific requirements.