We offer a complete end-to-end cell line and assay-development service.
Clonal cell line construction, assay integration, optimisation & validation
- Construction of recombinant clonal cell lines expressing a target of interest including:
- plasmid preparation & transfection / transduction
- antibiotic kill-curves
- antibiotic selection and cloning
- screening, clone selection and clone expansion
- Development and optimisation of cell-based assays including:
- integrating cell lines into assays involving fluorescence imaging and/or fluorescent/ bioluminescent plate-readers
- re-cloning to optimise expression levels
- continuous culture and assay-ready formats
- All cell lines are tested for sterility, cell growth, viability, and the absence of mycoplasma prior to shipping.
